- Act No 114/1992 Coll., on the Conservation of Nature and Landscape
- Act of 10 February 2004 No 100/2004 Coll., on the Conservation of Species of Wild Fauna and Flora, Regulated Trade Therein, and Other Measures to Protect Those Species, and on Amending Certain Acts (Act on Trade in Endangered Species)
- Act of 18 April 2003 No. 162/2003 Coll., on the conditions for the operation of zoological gardens and amendment to some laws (The Zoological Gardens Act)
- Act of 22 January 2004 No 78/2004 Coll., on the use of genetically modified organisms and genetic products
- Act No 115/2000 Coll., on compensation of damages caused by selected specially protected animals, as amended
- Act No 276/2003 Coll., on Antarctica
- Decree of the Government of the Czech Republic No 17/2004 Coll., on the rules for provision of subsidies to the operators of zoological gardens
- Decree of the Ministry of the Environment of 11 June 1992 No 395/1992 Coll., on implementing selected provisions of Czech National Council Act No. 114/1992 Coll. on the protection of the environment and the natural landscape
- Decree of the Ministry of the Environment of 15 April 2005 No 114/1992 Coll., on implementing certain provisions of Act No 114/1992 on conservation, as amended, in connection with the creation of the NATURA 2000 network
- Decree of 15 April 2004 No 209/2004 Coll., on detailed conditions for the use of genetically modified organisms and genetic products
- Decree No 210/2010 Coll., on implementing certain provisions of the Act on Trade in Endangered Species
- Decree No 29/2010 Coll., on further conditions for the use of genetically modified organisms and genetic
- Decree No 152/2006 Coll., on different procedure in the protection of birds and a derogation from basic conditions of protection of specially protected bird species for the purposes of their marking
- Decree No 294/2006 Coll., on different procedure in killing the Starling (Sturnus vulgaris)
- Decree No 360/2000 Coll., on the calculations of amount of compensation of damages caused by selected specially protected animals to specified domestic animals, guard dogs, fish, bee colonies, apiaries, unharvested field crops and forest stand,
- Decree No 316/2009 Coll., on keeping animals temporarily or permanently incapable of surviving in the wild and on the care of these animals in rescue stations (Decree on handicapped animals)